{"id":1563,"date":"2024-01-05T17:18:41","date_gmt":"2024-01-05T11:48:41","guid":{"rendered":"https:\/\/hindustan-medical.com\/e-shop\/?post_type=product&p=1563"},"modified":"2025-01-17T19:56:21","modified_gmt":"2025-01-17T14:26:21","slug":"quicksilver-powdered-buffer-packs","status":"publish","type":"product","link":"https:\/\/hindustan-medical.com\/e-shop\/product\/quicksilver-powdered-buffer-packs\/","title":{"rendered":"QuickSilver Powdered Buffer Packs"},"content":{"rendered":"
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Electrophoresis Buffers:<\/u><\/em><\/strong><\/h2>\n

TAE,\u00a0 Tris-Glycine-SDS, \u00a0Tris-MOPS-SDS, \u00a0Tris-MES-SDS<\/em><\/h3>\n

Western Blotting, ELISA and Cell Culture:<\/u><\/em><\/strong><\/h2>\n

TBS, TBS-T, PBS, PBS-T<\/em><\/h3>\n

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Nucleic Acid Electrophoresis Buffer<\/strong><\/h3>\n

QuickSilver\u2122 TAE (Tris-acetate-EDTA)<\/strong><\/p>\n

TAE is ideal for separation of large DNA fragments in agarose gels and is used as running buffer and in gel preparation.<\/p>\n

TAE is the buffer of choice when recovering DNA from gels and when downstream processing involves enzymatic reactions. Final concentration of a 1X working solution is 40mM Tris, 20mM acetic acid, 1mM EDTA.<\/p>\n

EB1000 QuickSilver TAE Buffer, 1X, 100 packs<\/p>\n

Protein Electrophoresis Buffers<\/strong><\/h3>\n

QuickSilver\u2122 TGS (Tris-Glycine-SDS)<\/strong><\/p>\n

Tris-Glycine-SDS is one of the most common running buffers used for SDS-PAGE. A 1X solution contains 25mM Tris, 192mM Glycine and 0.1% SDS.<\/p>\n

EB1100 QuickSilver Tris Glycine-SDS Buffer, 1X, 50 packs<\/p>\n

QuickSilver\u2122 Tris-MES-SDS<\/strong><\/p>\n

Small to medium molecular weight proteins can be resolved on SDS-PAGE gels using Tris-MES-SDS as a running buffer. Proteins resolve faster in gels using Tris-MES-SDS as a running buffer than those using Tris-MOPS-SDS. A 1X solution has a final concentration of 50mM Tris, 50mM MES, 0.1% SDS and 1mM EDTA.<\/p>\n

EB1101 QuickSilver Tris-MES-SDS Buffer, 1X, 50 packs<\/p>\n

QuickSilver\u2122 Tris-MOPS-SDS<\/strong><\/p>\n

Tris-MOPS-SDS is ideal for use as a running buffer when resolving medium to large proteins with SDS-PAGE. Gels run with Tris-MOPS-SDS buffer run slower than those using Tris-MES-SDS buffer. The final concentration of a 1X working solution is 50mM Tris, 50mM MOPS, 0.1% SDS and 1mM EDTA.<\/p>\n

EB1102 QuickSilver Tris-MOPS-SDS, 1X, 50 packs<\/p>\n

Western Blotting, ELISA, Cell Culture, Chromatography<\/strong><\/h3>\n

QuickSilver\u2122 PBS and PBST<\/strong><\/p>\n

Because its osmolality and ion concentration closely mimic that of the human body, PBS is commonly used for cell culture applications, as well as HPLC and MS. PBST is a wash buffer and diluent for ELISA. The 1X PBS solution contains 137mN NaCl, 2.7mM KCl and 10mM phosphate. PBST is supplemented with 0.05% Tween-20.<\/p>\n

EB1200 QuickSilver PBS Buffer, 1X, 100 packs<\/p>\n

EB1201 QuickSilver PBST Buffer, 1X, 100 packs<\/p>\n

QuickSilver\u2122 TBS and TBST<\/strong><\/p>\n

TBS and TBST are used to maintain pH within a very narrow range for a variety of applications including western blotting and immunoassays. The final concentration of a 1X working solution is 137mM NaCl, 2.7mM KCl, 50mM Tris. In addition, 1X TBST contains 0.1% Tween-20.<\/p>\n

EB1202 QuickSilver TBS buffer, 1X, 100 packs<\/p>\n

EB1203 QuickSilver TBST buffer, 1X, 100 packs<\/p>\n

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Ordering Information:<\/h2>\n\n\n\n\n\n\n\n\n\n\n\n\n
Item No.<\/th>\nDescription<\/th>\n<\/tr>\n<\/thead>\n
EB1000<\/td>\nQuickSilver TAE Buffer Powder, 100 pouches<\/td>\n<\/tr>\n
EB1100<\/td>\nQuickSilver Tris-Glycine-SDS Buffer Powder, 50 pouches<\/td>\n<\/tr>\n
EB1101<\/td>\nQuickSilver Tris-MES-SDS Buffer Powder, 50 pouches<\/td>\n<\/tr>\n
EB1102<\/td>\nQuickSilver Tris-MOPS-SDS Buffer Powder, 50 pouches<\/td>\n<\/tr>\n
EB1200<\/td>\nQuickSilver PBS Buffer Powder, 100 pouches<\/td>\n<\/tr>\n
EB1201<\/td>\nQuickSilver PBS-T Buffer Powder, 100 pouches<\/td>\n<\/tr>\n
EB1202<\/td>\nQuickSilver TBS Buffer Powder, 100 pouches<\/td>\n<\/tr>\n
EB1203<\/td>\nQuickSilver TBS-T Buffer Powder, 100 pouches<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n<\/div>\n
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Download the Catalogue<\/h2>\n