IsoPure™ 96

Automated System for Magnetic Bead-Based Purification

  • Compatible with all popular magnetic bead kits

  • Touch screen panel for on-board programming and operation

  • Process up to 96 samples in deep-well plates

  • 8-position carousel with 2 heating blocks

  • Sample volume 50µl – 1000µl

Description

The IsoPure™ 96 from Accuris Instruments is a high throughput, automated system that improves the efficiency and reproducibility of magnetic bead-based purification protocols. Capable of processing up to 96 samples at one time, the instrument is easy to set up and program. Mixing, magnetic bead transfer, washing, and elution steps are performed automatically. The purification process can be completed within 10 to 60 minutes depending on the type of reagent kit used and complexity of the program.

 

 

 

 

 

 

Programming

The IsoPure™ 96 can be programmed by using the color touch screen or the included mouse. After a protocol has been created, the instrument will calculate and display the estimated time for each step and the total time.

Typical step settings include:

Tip Comb Loading and Unloading:   Automate tip comb loading and unloading by designating the respective plate positions in the script.

Mixing:  Parameters for mixing time, speed, and amplitude can be programmed for each step of the protocol. 

Temperature Control:  Heating of the sample mixture during the lysis and elution steps significantly improves the purity and yield of the target molecule. The IsoPure™ 96 is equipped with 2 heating blocks on the plate carousel that can be precisely set to temperatures from ambient + 5°C to 120°C. IsoPure™ deep well plates fit into the heating blocks for efficient heat transfer. A cooling fan inside the processing chamber maintains the ambient operating temperature to ensure consistent results.

Bead Collection and Release:  The complete collection of magnetic beads from sample mixtures is crucial for maximizing the yield of purified analytes. The IsoPure™ software offers full control of magnetic parameters to ensure cleaner extractions.  General settings allow control of magnet speed, position, repeat collection cycles, etc, to guarantee consistent yields.

 

Script Optimization

Accuris Instruments has partnered with leading magnetic bead kit manufacturers to develop and optimize protocols for specific applications. To request scripts for specific kits, or for assistance in optimization, please contact the IsoPure™ Technical Support Team at info@accuris-usa.com.

 

Built-in UV Sterilization

An ultraviolet sterilization lamp (UVC) inside the processing chamber provides effective elimination of most bacterial, viral and stray genomic DNA contaminants.  This additional safeguard reduces the possibility of unwanted DNA/RNA contamination of critical samples that could be amplified downstream in a PCR detection assay. UV sterilization time can be adjusted based on specific program requirements to ensure a sterile environment prior to purification.

 

Connection Ports 

The IsoPure™ 96 can store up to 100 program files which can be transferred between instruments, or to a computer for storage or sharing. Simply insert the included flash drive to export the selected files. Programming control can be done via the touch screen, or by using the included mouse. A PC can also be connected for remote programming.

 

IsoPure™ Consumables

Accuris offers high-quality deep well plates and magnetic tip combs designed to fit the IsoPure™ 96. Produced using virgin polypropylene and packaged in a sterile facility, these consumables are guaranteed to be contaminant-free. The IsoPure™ 96 also accepts industry-standard 96 deep well plates and tip combs designed for high throughput purification systems.

Specifications

Capacity / sample volume 96 samples  /  50µL to 1000µL
Display 7.0 inch-color touch screen
Plate positions 8 Plate positions
Lysis temperature range Ambient +5°C to 120°C
Elution temperature range Ambient +5°C to 120°C
Mixing speed Variable, 10 levels
Programming control Touch screen/mouse or PC connection
Program memory 100 programs (internal memory)
Communication ports USB, LAN, & PC
Magnetic bead recovery rate >95%
Internal lighting LED
Contamination control UV lamp (3W, 253.7nm UVC wavelength)
Dimensions 21.6 x 24.4 x 18.9 in. (55 x 62 x 48 cm)
Weight 54 kg / 119 lbs
Electrical input Universal 120 to 240VAC
Warranty 2 years

Ordering Information:

Cat No. Description
AP1096 IsoPure 96, Automated Extraction Instrument
AP1096-DWP 96 deep well plates for IsoPure 96, cs 50
AP1096-ELP 96 well elution plates for IsoPure™, cs 100
AP1096-TC Tip Comb for IsoPure 96, cs 50

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